type rosα against m luteus atcc 10240  (ATCC)

Journal: Applied and Environmental Microbiology

Article Title: Modifying the Lantibiotic Mutacin 1140 for Increased Yield, Activity, and Stability

doi: 10.1128/AEM.00830-18

Figure Lengend Snippet: Summary of the mutacin 1140 core peptide mutants generated in this study. Single core peptide amino acid substitutions and multiple amino acid substitutions in the hinge region are shown above the provided mutacin 1140 sequence, while multiple substitutions in both the N-terminal and hinge regions are shown below the mutacin 1140 sequence. Each lane represents one single core peptide mutant. Bold amino acid abbreviations represent mutant strains with significantly increased bioactivity, gray abbreviations represent mutant strains with significantly decreased bioactivity, and underlined abbreviations represent mutant strains that are inactive. The rest of the mutant strains presented had no statistically significant change in bioactivity against the indicator strain M. luteus ATCC 10240.

Article Snippet: This analog had the same inhibitory activity as that of the wild type against M. luteus and a 2-fold increase in activity against S. pneumoniae ATCC 27336.

Techniques: Generated, Sequencing, Mutagenesis

Journal: Applied and Environmental Microbiology

Article Title: Modifying the Lantibiotic Mutacin 1140 for Increased Yield, Activity, and Stability

doi: 10.1128/AEM.00830-18

Figure Lengend Snippet: Bioactivities of core peptide (mutA) mutant strains compared to that of wild-type S. mutans JH1140. (A) Single point mutations in the core peptide. (B) Multiple amino acid substitution mutations in the hinge region. (C) Multiple amino acid substitution mutations within the N-terminal and hinge regions. The bioactivities of the mutants were measured as the ratio of the area of the zone of inhibition of each mutant strain to the area of the zone of inhibition of the wild-type strain. A value of >1.0 indicates that the mutant strain is more active than the wild-type strain. M. luteus ATCC 10240 was used as the indicator strain for antimicrobial activity assays, and experiments were done in triplicate. The Student t test was used for statistical analysis, and asterisks signify statistical significance (P < 0.05).

Article Snippet: This analog had the same inhibitory activity as that of the wild type against M. luteus and a 2-fold increase in activity against S. pneumoniae ATCC 27336.

Techniques: Mutagenesis, Inhibition, Activity Assay

Journal: Applied and Environmental Microbiology

Article Title: Modifying the Lantibiotic Mutacin 1140 for Increased Yield, Activity, and Stability

doi: 10.1128/AEM.00830-18

Figure Lengend Snippet: MICs and MLCs (μg/ml) of mutacin 1140 analogs against four indicator strains

Article Snippet: This analog had the same inhibitory activity as that of the wild type against M. luteus and a 2-fold increase in activity against S. pneumoniae ATCC 27336.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Modifying the Lantibiotic Mutacin 1140 for Increased Yield, Activity, and Stability

doi: 10.1128/AEM.00830-18

Figure Lengend Snippet: Masses of mutacin 1140 analogs generated by thiol organosulfur labeling and bioactivities against selected bacteria

Article Snippet: This analog had the same inhibitory activity as that of the wild type against M. luteus and a 2-fold increase in activity against S. pneumoniae ATCC 27336.

Techniques: Generated, Labeling

Journal: Applied and Environmental Microbiology

Article Title: Modifying the Lantibiotic Mutacin 1140 for Increased Yield, Activity, and Stability

doi: 10.1128/AEM.00830-18

Figure Lengend Snippet: Trypsin stability of selected mutacin 1140 analogs. Wild-type mutacin 1140 and the K2A, R13A, K2A:R13A, T14A, and S5A:T14G mutants were tested. The bioactivity following trypsin digestion was measured as the ratio of the areas of the zones of inhibition of the trypsin-treated analog and the untreated analog. A value of 1.0 would indicate that the analog retained 100% of activity following trypsin exposure. M. luteus ATCC 10240 was used as the indicator strain for antimicrobial activity assays. The experiments were done in duplicate.

Article Snippet: This analog had the same inhibitory activity as that of the wild type against M. luteus and a 2-fold increase in activity against S. pneumoniae ATCC 27336.

Techniques: Inhibition, Activity Assay

Journal: Applied and Environmental Microbiology

Article Title: Modifying the Lantibiotic Mutacin 1140 for Increased Yield, Activity, and Stability

doi: 10.1128/AEM.00830-18

Figure Lengend Snippet: Strains used in this study

Article Snippet: This analog had the same inhibitory activity as that of the wild type against M. luteus and a 2-fold increase in activity against S. pneumoniae ATCC 27336.

Techniques: Cloning, Overlay Assay

Journal: Applied and Environmental Microbiology

Article Title: Site-Directed Mutations in the Lanthipeptide Mutacin 1140

doi: 10.1128/AEM.00704-13

Figure Lengend Snippet: Bioactivity of mutacin 1140 variants. (A) Growth inhibition of the intertwined rings C and D and N-terminal mutants in comparison to the wild-type strain. (B) Growth inhibition of ring A and hinge region mutants in comparison to the wild-type strain. (C) Relative activity of each mutant with respect to the wild-type area (mm2) of inhibition. Micrococcus luteus ATCC 10240 was used as the indicator strain for antimicrobial activity.

Article Snippet: Mu1140 Phe1Ile had a 4-fold increase in activity over that of wild-type mutacin 1140 against M. luteus ATCC 10240; the values observed were 0.0156 μg/ml and 0.625 μg/ml for the wild type and mu1140 Phe1Ile, respectively ( ).

Techniques: Inhibition, Comparison, Activity Assay, Mutagenesis

Journal: Applied and Environmental Microbiology

Article Title: Site-Directed Mutations in the Lanthipeptide Mutacin 1140

doi: 10.1128/AEM.00704-13

Figure Lengend Snippet: MICs for mutacin 1140 and structural variants

Article Snippet: Mu1140 Phe1Ile had a 4-fold increase in activity over that of wild-type mutacin 1140 against M. luteus ATCC 10240; the values observed were 0.0156 μg/ml and 0.625 μg/ml for the wild type and mu1140 Phe1Ile, respectively ( ).

Techniques: Variant Assay